PXD033305 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis |
Description | Sexual reproduction in angiosperms requires the production and delivery of two male gametes by a three-celled haploid male gametophyte. This demands synchronised gene expression in a short developmental window to ensure double fertilization and seed set. While transcriptomic changes in developing pollen are known for Arabidopsis, no studies have integrated RNA and proteomic data in this model. Further, the role of alternative splicing has not been fully addressed, yet post-transcriptional and post-translational regulation may have a key role in gene expression dynamics during microgametogenesis. We have refined and substantially updated global transcriptomic and proteomic changes in developing pollen for two Arabidopsis accessions. Despite the superiority of RNA-seq over microarray-based platforms, we demonstrate high reproducibility and comparability. We identify thousands of long non-coding RNAs as potential regulators of pollen development, hundreds of changes in alternative splicing and provide insight into mRNA translation rate and storage in developing pollen. Our analysis delivers an integrated perspective of gene expression dynamics in developing Arabidopsis pollen and a foundation for studying the role of alternative splicing in this model. |
HostingRepository | PRIDE |
AnnounceDate | 2022-11-01 |
AnnouncementXML | Submission_2022-11-01_03:58:13.894.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | DavidPotesil |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-04-19 06:55:08 | ID requested | |
⏵ 1 | 2022-11-01 03:58:14 | announced | |
2 | 2023-11-14 08:55:39 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Arabidopsis, Ler-0, LC-MS, male gametophyte, microgametogenesis, Col-0, proteome, RNA-seq |
Contact List
ZbynekZdrahal |
contact affiliation | Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic |
contact email | zdrahal@sci.muni.cz |
lab head | |
DavidPotesil |
contact affiliation | Proteomics Core Facility and Research Group Proteomics CEITEC-MU Masaryk University Brno, Czech Republic |
contact email | david.potesil@ceitec.muni.cz |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD033305 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033305
- Label: PRIDE project
- Name: Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis