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PXD033250-2

PXD033250 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleVirulence induction in Pseudomonas aeruginosa under inorganic phosphate limitation: a proteomics perspective
DescriptionInorganic phosphate (Pi) is a central nutrient and signal molecule for bacteria. Pi limitation was shown to increase the virulence of a number of phylogenetically diverse pathogenic bacteria with different in lifestyles. Hypophosphatemia enhances the risk of death in patients due to general bacteremia and was observed after the surgical injury in humans and animals. Phosphate therapy, or the reduction of bacterial virulence by the administration of Pi or phosphate-containing compounds, is a promising anti-infective therapy approach that will not cause cytotoxicity nor the emergence of antibiotic-resistant strains. The proof of concept of phosphate therapy has been obtained using primarily Pseudomonas aeruginosa (PA) as a model. However, a detailed understanding of Pi-induced changes at protein levels is missing. Using pyocyanin production as proxy, we show that the Pi-mediated induction of virulence is a highly cooperative process that occurs between 0.2 to 0.6 mM Pi. We present a proteomics study of PA grown in minimal medium supplemented with either 0.2 or 1 mM Pi and rich medium. About half of the predicted PA proteins could be quantified. Among the 1,471 dysregulated proteins comparing growth in 0.2 mM to 1 mM Pi, 1100 were depleted under Pi-deficient conditions. Most of these proteins are involved in general and energy metabolism, different biosynthetic and catabolic routes, or transport. Pi depletion caused accumulation of proteins that belong to all major families of virulence factor categories, including pyocyanin synthesis, secretion systems, quorum sensing, chemosensory signaling and the secretion of proteases, phospholipases and phosphatases.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:55:37.533.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSandra Maass
SpeciesList scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationListacetylated residue; monohydroxylated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-14 05:43:02ID requested
12022-11-02 10:46:36announced
22023-11-14 08:55:37announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: anti-infective therapy, proteomics, virulence,Pseudomonas aeruginosa, pyocyanin, phosphate starvation, quorum sensing
Contact List
Dörte Becher
contact affiliationDepartment of Microbial Proteomics, Institute of Microbiology, University of Greifswald, D-17489 Greifswald, Germany
contact emaildbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliationUniversity of Greifswald, Department for Microbial Proteomics
contact emailsandra.maass@uni-greifswald.de
dataset submitter
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