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PXD033234-1

PXD033234 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCryoEM structure of mitochondrial complex I from the thermophilic eukaryote Chaetomium thermophilum
DescriptionMitochondrial complex I is a redox-driven proton pump that generates most of the proton-motive force powering oxidative phosphorylation and ATP synthesis in eukaryotes. We report the structure of complex I from the thermophilic eukaryote Chaetomium thermophilum, determined by electron cryo-microscopy to 2.4 A resolution in the open and closed conformation. Complex I has two arms, the peripheral and membrane arm, forming an L-shape. The two conformations differ in the relative position of the two arms. The open-to-closed transition is accompanied by substantial conformational changes in the Q-binding cavity and the E-channel, and by the formation of an aqueous connection between the E-channel and an extensive aqueous passage inside the membrane arm. The observed similarities provide strong support for a conserved, common mechanism that applies across all species from fungi to mammals. Furthermore, the complex is inhibited by the detergent DDM, which binds reversibly to two sites in the Q-binding cavity.
HostingRepositoryPRIDE
AnnounceDate2022-11-29
AnnouncementXMLSubmission_2022-11-29_01:34:30.156.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJulianLanger
SpeciesList scientific name: Chaetomium thermophilum; NCBI TaxID: 209285;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-14 05:07:32ID requested
12022-11-29 01:34:30announced
22023-11-14 08:54:13announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: mitochondrial complex I, Chaetomium thermophilum
Contact List
JulianLanger
contact affiliationMax Planck Institute of Biophysics, Max Planck Institute for Brain Research
contact emailjulian.langer@biophys.mpg.de
lab head
JulianLanger
contact affiliationMPIs for Biophysics and Brain Research
contact emailjulian.langer@biophys.mpg.de
dataset submitter
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