<<< Full experiment listing

PXD033226-2

PXD033226 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEnrichment and Fragmentation Approaches for Enhanced Detection and Characterization of Endogenous Glycosylated Neuropeptides
DescriptionGlycosylation, a common and heterogeneous post-translational modification plays a key role in altering biomolecule function and other properties. Prior to monitoring regulatory roles, basal glyconeuropeptide expression must be determined. Characterization is partially limited by the lower abundance of glycosylated neuropeptides in vivo compared to non-modified neuropeptides and thus requires effective enrichment and characterization strategies. A hydrophilic interaction liquid chromatography enrichment strategy is modified, and fragmentation schemes are evaluated to establish a workflow for future glyconeuropeptide studies to improve the understanding of neuropeptide glycosylation, as well as its distribution across the neuroendocrine system and several neuropeptide families. Product ion-triggered electron-transfer/higher-energy collision dissociation and stepped collision energy higher-energy collisional dissociation are evaluated.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:54:12.571.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAshley Phetsanthad
SpeciesList scientific name: Callinectes sapidus; NCBI TaxID: 6763;
ModificationListamidated residue; glycosylated residue
InstrumentOrbitrap Fusion Lumos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-14 04:29:08ID requested
12022-08-19 13:19:53announced
22023-11-14 08:54:13announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: glycosylation, neuropeptides, endogenous peptides, mass spectrometry,de novo sequencing
Contact List
Lingjun Li
contact affiliationSchool of Pharmacy and Department of Chemistry, Lingjun Li Lab, University of Wisconsin - Madison, USA
contact emaillingjun.li@wisc.edu
lab head
Ashley Phetsanthad
contact affiliationUniversity of Wisconsin - Madison
contact emailashphets@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD033226
PRIDE project URI
Repository Record List
[ + ]