PXD033064-1

PXD033064 is an original dataset announced via ProteomeXchange.

Title	Mitochondria regulate intracellular coenzyme Q transport and ferroptotic resistance via STARD7
Description	Coenzyme Q (or ubiquinone) is a redox-active lipid, which serves as universal electron carrier in the mitochondrial respiratory chain and antioxidant in the plasma membrane limiting lipid peroxidation and ferroptosis. Mechanisms allowing cellular coenzyme Q distribution after synthesis within mitochondria are not understood. Here, we identify the cytosolic lipid transfer protein STARD7 as a critical and limiting factor of intracellular coenzyme Q transport. Dual localization of STARD7 to the intermembrane space of mitochondria and the cytosol upon cleavage by the rhomboid protease PARL ensures the synthesis of coenzyme Q in mitochondria and its transport to the plasma membrane. While cytosolic STARD7 overexpression protects against ferroptosis, it limits CoQ abundance in mitochondria and respiratory cell growth, illustrating the need to coordinate CoQ synthesis and cellular distribution by PARL-mediated STARD7 processing. Our findings highlight the antioxidant function of mitochondrial CoQ against ferroptosis and identify PARL and STARD7 as promising targets to interfere with ferroptosis. The repository contains raw files and methods for project 0112 (brain – whole proteomics) and 0176 (spatial proteomics). The labeling of the raw files and the experiment – raw file matches are available in the individual search zip files. The individual sections are accompanied with the project identifier.
HostingRepository	PRIDE
AnnounceDate	2022-11-12
AnnouncementXML	Submission_2022-11-12_05:58:46.850.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	HendrikNolte
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-07 03:30:02	ID requested
⏵ 1	2022-11-12 05:58:47	announced

Dataset with its publication pending

submitter keyword: Mitochondria,Stard7, CoQ

ThomasLanger
contact affiliation	Max-Planck-Institute for Biology of Ageing Joseph-Stelzmann-Str. 9b 50931 Cologne, Germany
contact email	tlanger@age.mpg.de
lab head
HendrikNolte
contact affiliation	Max Planck Institute for Biology of Ageing, 50931 Cologne, Germany
contact email	h.nolte@age.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD033064

PRIDE project URI

• PRIDE
  1. PXD033064
     1. Label: PRIDE project
     2. Name: Mitochondria regulate intracellular coenzyme Q transport and ferroptotic resistance via STARD7