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PXD033045-1

PXD033045 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNucleoporins facilitate ORC loading onto chromatin
DescriptionThe origin recognition complex (ORC) binds throughout the genome to initiate DNA replication. In metazoans, it is still unclear how ORC is targeted to specific loci to facilitate helicase loading and replication initiation. Here, we performed immunoprecipitations coupled with mass spectrometry for ORC2 in Drosophila embryos. Surprisingly, we found that ORC2 associates with multiple subunits of the Nup107-160 subcomplex of the nuclear pore. Bioinformatic analysis revealed that, relative to all modENCODE factors, nucleoporins are among the most enriched factors at ORC2 binding sites. Critically, depletion of the nucleoporin Elys, a member of the Nup107-160 complex, results in decreased ORC2 loading onto chromatin. Depleting Elys also sensitized cells to replication fork stalling, which could reflect a defect in establishing dormant replication origins. Our work reveals a new connection between ORC, replication initiation and nucleoporins, highlighting a previously unrecognized function of nucleoporins in metazoan replication initiation.
HostingRepositoryPRIDE
AnnounceDate2022-10-19
AnnouncementXMLSubmission_2022-10-19_09:51:46.941.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLoganRichards
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-07 02:55:06ID requested
12022-10-19 09:51:47announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: DNA replication, Drosophila, genome stability, Nuclear Pore
Contact List
JaredNordman
contact affiliationDepartment of Biological Sciences, Vanderbilt University
contact emailjared.nordman@vanderbilt.edu
lab head
LoganRichards
contact affiliationVanderbilt University
contact emaillogan.r.richards@vanderbilt.edu
dataset submitter
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Dataset FTP location
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