PXD033015-1
PXD033015 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | SARS-CoV-2 phosphoproteomics - ACE2-A549 and Vero |
Description | Multiple coronavirus have emerged independently in the past 20 years and caused lethal human diseases. Although vaccine development targeting these viruses has been accelerated substantially, there remain patients requiring treatment who cannot be vaccinated. Understanding the common host factors necessary for the life cycle of coronaviruses may reveal conserved therapeutic targets. Here, we used the known substrate specificities of mammalian protein kinases to deconvolute the sequence of phosphorylation events mediated by three host protein kinase families (SRPK, GSK-3, and CK1) that phosphorylate a cluster of serine and threonine residues on the viral N-protein in a highly coordinated way. We showed that these kinases were critical for the viral replication cycle, suggesting that inhibitors of one or more of these protein kinases could be useful for treating patients with COVID-19 infections. These phosphorylation sites are highly conserved across coronaviruses, indicating that inhibitors of these protein kinases might be broadly effective in treating multiple viral diseases. |
HostingRepository | PRIDE |
AnnounceDate | 2023-03-11 |
AnnouncementXML | Submission_2023-03-11_03:57:37.767.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | TomerYaron |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Chlorocebus sabaeus; NCBI TaxID: 60711; |
ModificationList | phosphorylated residue; monohydroxylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-04-06 08:20:00 | ID requested | |
⏵ 1 | 2023-03-11 03:57:38 | announced | |
2 | 2023-11-14 08:42:43 | announced | 2023-11-14: Updated project metadata. |
Publication List
Yaron TM, Heaton BE, Levy TM, Johnson JL, Jordan TX, Cohen BM, Kerelsky A, Lin TY, Liberatore KM, Bulaon DK, Van Nest SJ, Koundouros N, Kastenhuber ER, Mercadante MN, Shobana-Ganesh K, He L, Schwartz RE, Chen S, Weinstein H, Elemento O, Piskounova E, Nilsson-Payant BE, Lee G, Trimarco JD, Burke KN, Hamele CE, Chaparian RR, Harding AT, Tata A, Zhu X, Tata PR, Smith CM, Possemato AP, Tkachev SL, Hornbeck PV, Beausoleil SA, Anand SK, Aguet F, Getz G, Davidson AD, Heesom K, Kavanagh-Williamson M, Matthews DA, tenOever BR, Cantley LC, Blenis J, Heaton NS, Host protein kinases required for SARS-CoV-2 nucleocapsid phosphorylation and viral replication. Sci Signal, 15(757):eabm0808(2022) [pubmed] |
Keyword List
ProteomeXchange project tag: Sars-cov-2, Covid-19 |
submitter keyword: COVID-19, SARS-CoV-2, phosphoproteomics |
Contact List
Lewis C.Cantley | |
---|---|
contact affiliation | Department of Medicine, Weill Cornell Medicine, New York, NY, USA |
contact email | LCantley@med.cornell.edu |
lab head | |
TomerYaron | |
contact affiliation | Weill Cornell Medicine |
contact email | toy2005@med.cornell.edu |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD033015 |
PRIDE project URI |
Repository Record List
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