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PXD033012-1

PXD033012 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRegionally defined proteomic profiles of human cerebral tissue and organoids reveal conserved molecular modules of neurodevelopment
DescriptionCerebral organoids have emerged as faithful humanoid avatars for modeling neurodevelopmental and pathological processes. Additionally, they serve as powerful discovery platforms for less characterized neurobiological programs. Towards this prospect, we leveraged mass spectrometry-based proteomics to molecularly profile precursor and neural compartments of both human-derived organoids and mid-gestation fetal brain tissue, to define overlapping programs. Our analysis included precursor-enriched transcriptional regulatory proteins that were not found to be differentially expressed in previous transcriptomic datasets. To highlight the discovery potential of this resource, we show that RUVBL2 is preferentially expressed in the SOX2-positive compartment of organoids and chemical inactivation leads to precursor cell displacement and apoptosis. To explore clinicopathological correlates of this cytoarchitectural disruption, we interrogated clinical datasets, and identified rare de novo genetic variants involving RUVBL2 in patients with neurodevelopmental impairments. Together, our study demonstrates how cell-type specific profiling of organoids can help nominate previously unappreciated genes in neurodevelopment and disease.
HostingRepositoryPRIDE
AnnounceDate2022-04-07
AnnouncementXMLSubmission_2022-04-07_03:35:25.729.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterUgljesa Djuric
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-06 08:12:19ID requested
12022-04-07 03:35:26announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: neurodevelopment, proteomics, mass spectrometry
Contact List
Phedias Diamandis
contact affiliationPrincess Margaret Hospital University of Toronto University Health Network
contact emailphedias.diamandis@uhn.ca
lab head
Ugljesa Djuric
contact affiliationMt. Sinai Hospital
contact emailugljesa.djuric@mail.utoronto.ca
dataset submitter
Full Dataset Link List
Dataset FTP location
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