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PXD033010-2

PXD033010 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLung macrophages utilize unique cathepsin K-dependent phagosomal machinery to degrade intracellular collagen
DescriptionResident tissue macrophages (RTMs) are organ-specialized phagocytes responsible for the maintenance and protection of residing tissue. It is well established that tissue diversity is reflected by the heterogeneity of RTMs origin and phenotype. However, much less is known about tissue-specific phagocytic macrophage functions. Here, using quantitative proteomics approach, we identify cathepsins as key determinants of phagosome maturation in primary peritoneal, lung and brain resident macrophages. The data further uncover cathepsin K (CtsK) as a molecular marker for lung phagosomes required for intracellular protein and collagen degradation. Pharmacological blockade of CtsK activity diminished phagosomal proteolysis and collagenolysis in lung resident macrophages. Furthermore, pro-fibrotic TGF-β negatively regulated CtsK-mediated phagosomal collagen degradation independently from classical endocytic proteolytic pathways. In humans, phagosomal CtsK activity was reduced in lung COPD macrophages and lung macrophages exposed to cigarette smoke extract. Taken together, this study provides a comprehensive map of how peritoneal, lung and brain tissue environment shapes phagosomal composition of resident macrophages, revealing CtsK as a key molecular determinant of lung phagosomes contributing to phagocytic collagen clearance in lungs.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:55:20.215.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIvo Fabrik
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-06 08:11:06ID requested
12023-01-17 23:47:38announced
22023-11-14 08:55:20announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: phagocytosis, proteomics,Cathepsins, resident tissue macrophages, phagosome maturation
Contact List
Anetta Härtlova
contact affiliationInstitute of Biomedicine, Department of Microbiology, the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
contact emailanetta.hartlova@gu.se
lab head
Ivo Fabrik
contact affiliationBiomedical Research Center, University Hospital Hradec Kralove
contact emailivo.fabrik@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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