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PXD033006-1

PXD033006 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePlacental macrophage (Hofbauer cell) response to viral and bacterial ligands is influenced by fetal sex
DescriptionBacterial and viral infections of the placenta are associated with inflammation and adverse pregnancy outcomes. Hofbauer cells (HBCs) are specialised fetal-origin macrophages in the placental villi and are proposed to protect the fetus from vertical transmission of pathogens; however, they are poorly understood. Here, we have performed quantitative proteomics on term HBCs under resting conditions and following exposure to bacterial and viral pathogen associated molecular patterns (PAMPs), and investigated the contribution of fetal sex to these responses. Resting HBCs expressed a plethora of proteins pertinent to macrophage function, including chemokines, cytokines, Toll-like receptors, and classical and non-classical major histocompatibility complex class I and II molecules. HBCs mounted divergent responses to bacterial versus viral PAMPs but exhibited protein expression changes suggestive of a switch towards a more pro-inflammatory phenotype. A comparison between male and female HBCs, showed that the latter mounted a much stronger and wider response. Sexual dimorphism in HBCs was primarily associated with lipid metabolism in males and cytoskeleton organisation in females. We provide a novel and comprehensive understanding regarding the phenotype of term placental macrophages and their sex-dependent responses to infectious triggers.
HostingRepositoryPRIDE
AnnounceDate2022-10-31
AnnouncementXMLSubmission_2022-10-31_05:29:46.201.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPaschaliaPantazi
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-06 08:04:46ID requested
12022-10-31 05:29:46announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: placental macrophages, Hofbauer cells, nano LC-MSMS,human
Contact List
BethHolder
contact affiliationDept of Metabolism Digestion and Reproduction, Imperial College London, London, UK
contact emailb.holder@imperial.ac.uk
lab head
PaschaliaPantazi
contact affiliationImperial College London
contact emailp.pantazi@imperial.ac.uk
dataset submitter
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Dataset FTP location
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