PXD032977

PXD032977 is an original dataset announced via ProteomeXchange.

Title	Yeast ORC sumoylation status fine-tunes origin activity
Description	Sumoylation is emerging as a post-translation modification important for chromosome duplication and stability. The origin recognition complex (ORC), which directs DNA replication initiation by loading the MCM replicative helicases onto origins, is sumoylated in both yeast and human cells. However, the biological consequences of ORC sumoylation are largely unclear. Here we report the effects of hyper- and hypo-sumoylation of yeast ORC using multiple approaches. We show that ORC hyper-sumoylation preferentially reduces the activity of a subset of early origins, while Orc2 hypo-sumoylation has an opposing effect. Mechanistically, ORC hyper-sumoylation leads to reduced MCM loading in vitro and diminished MCM chromatin association in vivo. The importance of an appropriate level of ORC sumoylation is suggested by the data that either hyper- or hypo-sumoylation of ORC results in genome instability and a dependence on other genome maintenance factors for cell fitness. Thus, yeast ORC sumoylation status needs to be fine-tuned to achieve optimal origin activity control and genome stability.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:42:07.234.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gemma Regan-Mochrie
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	sumoylated lysine
Instrument	ultraflex

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-03 08:12:23	ID requested
1	2023-03-11 03:39:18	announced
⏵ 2	2023-11-14 08:42:08	announced	2023-11-14: Updated project metadata.

Regan-Mochrie G, Hoggard T, Bhagwat N, Lynch G, Hunter N, Remus D, Fox CA, Zhao X, Yeast ORC sumoylation status fine-tunes origin licensing. Genes Dev, 36(13-14):807-21(2022) [pubmed]

submitter keyword: Orc2, SUMO,yeast

Xiaolan Zhao
contact affiliation	Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, USA
contact email	zhaox1@mskcc.org
lab head
Gemma Regan-Mochrie
contact affiliation	Memorial Sloan Kettering Cancer Center
contact email	reganmog@mskcc.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD032977

PRIDE project URI

• PRIDE
  1. PXD032977
     1. Label: PRIDE project
     2. Name: Yeast ORC sumoylation status fine-tunes origin activity