PXD032928 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterization of pathogenic Candida glabrata and their effectiveness against systemic candidiasis in BALB/c mice |
Description | Candida glabrata is a common opportunistic fungal pathogen of humans, with the emergence of resitant strains being of particulary concern. The cell wall associated and secretory proteins are intrinsically involved in vital functions during the fungal-host interactions. These proteins are responsible for eliciting immune response in host during candidiasis and therefore can be a promising candidates for vaccine development. BALB/c mice were broadly divided into unimmunized, immunized groups and the designated groups were immunized with β-mercaptoethanol (β-ME) call wall extract, and secretory protein extract from C. glabrata via intraperitoneal route. All the animals were then challenged with a lethal dose of C. glabrata. Furthermore, we applied a liquid chromatography-tandem mass spectrometry (LC–MS/MS) -based approach for the characterization of β-ME extract and secretory protein extract from C. glabrata, which resulted in a comprehensive cell wall and secretory proteome map.portunistic fungal pathogen of humans, with the emergence of resitant strains being of particulary concern. The cell wall associated and secretory proteins are intrinsically involved in vital functions during the fungal-host interactions. These proteins are responsible for eliciting immune response in host during candidiasis and therefore can be a promising candidates for vaccine development. BALB/c mice were broadly divided into unimmunized, immunized groups and the designated groups were immunized with β-mercaptoethanol (β-ME) call wall extract, and secretory protein extract from C. glabrata via intraperitoneal route. All the animals were then challenged with a lethal dose of C. glabrata. Furthermore, we applied a liquid chromatography-tandem mass spectrometry (LC–MS/MS) -based approach for the characterization of β-ME extract and secretory protein extract from C. glabrata, which resulted in a comprehensive cell wall and secretory proteome map. |
HostingRepository | PRIDE |
AnnounceDate | 2023-03-11 |
AnnouncementXML | Submission_2023-03-11_03:29:41.114.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | PrevinNaicker |
SpeciesList | scientific name: Candida glabrata (Yeast) (Torulopsis glabrata); NCBI TaxID: 5478; |
ModificationList | iodoacetamide derivatized residue |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-04-02 12:58:06 | ID requested | |
⏵ 1 | 2023-03-11 03:29:42 | announced | |
2 | 2023-11-14 08:41:53 | announced | 2023-11-14: Updated project metadata. |
Publication List
Kamli MR, Sabir JSM, Malik MA, Ahmad A, and Their Effectiveness against Systemic Candidiasis in BALB/c Mice for Vaccine Development. Pharmaceutics, 14(10):(2022) [pubmed] |
Keyword List
submitter keyword: LC-MS, Mouse model,Candida glabrata |
Contact List
StoyanStoychev |
contact affiliation | NextGen Health CSIR South Africa |
contact email | sstoychev@csir.co.za |
lab head | |
PrevinNaicker |
contact affiliation | Council for Scientific and Industrial Research |
contact email | pnaicker1@csir.co.za |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD032928
- Label: PRIDE project
- Name: Characterization of pathogenic Candida glabrata and their effectiveness against systemic candidiasis in BALB/c mice