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PXD032928

PXD032928 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCharacterization of pathogenic Candida glabrata and their effectiveness against systemic candidiasis in BALB/c mice
DescriptionCandida glabrata is a common opportunistic fungal pathogen of humans, with the emergence of resitant strains being of particulary concern. The cell wall associated and secretory proteins are intrinsically involved in vital functions during the fungal-host interactions. These proteins are responsible for eliciting immune response in host during candidiasis and therefore can be a promising candidates for vaccine development. BALB/c mice were broadly divided into unimmunized, immunized groups and the designated groups were immunized with β-mercaptoethanol (β-ME) call wall extract, and secretory protein extract from C. glabrata via intraperitoneal route. All the animals were then challenged with a lethal dose of C. glabrata. Furthermore, we applied a liquid chromatography-tandem mass spectrometry (LC–MS/MS) -based approach for the characterization of β-ME extract and secretory protein extract from C. glabrata, which resulted in a comprehensive cell wall and secretory proteome map.portunistic fungal pathogen of humans, with the emergence of resitant strains being of particulary concern. The cell wall associated and secretory proteins are intrinsically involved in vital functions during the fungal-host interactions. These proteins are responsible for eliciting immune response in host during candidiasis and therefore can be a promising candidates for vaccine development. BALB/c mice were broadly divided into unimmunized, immunized groups and the designated groups were immunized with β-mercaptoethanol (β-ME) call wall extract, and secretory protein extract from C. glabrata via intraperitoneal route. All the animals were then challenged with a lethal dose of C. glabrata. Furthermore, we applied a liquid chromatography-tandem mass spectrometry (LC–MS/MS) -based approach for the characterization of β-ME extract and secretory protein extract from C. glabrata, which resulted in a comprehensive cell wall and secretory proteome map.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:41:52.500.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPrevin Naicker
SpeciesList scientific name: Candida glabrata (Yeast) (Torulopsis glabrata); NCBI TaxID: 5478;
ModificationListiodoacetamide derivatized residue
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-02 12:58:06ID requested
12023-03-11 03:29:42announced
22023-11-14 08:41:53announced2023-11-14: Updated project metadata.
Publication List
Kamli MR, Sabir JSM, Malik MA, Ahmad A, and Their Effectiveness against Systemic Candidiasis in BALB/c Mice for Vaccine Development. Pharmaceutics, 14(10):(2022) [pubmed]
Keyword List
submitter keyword: LC-MS, Mouse model,Candida glabrata
Contact List
Stoyan Stoychev
contact affiliationNextGen Health CSIR South Africa
contact emailsstoychev@csir.co.za
lab head
Previn Naicker
contact affiliationCouncil for Scientific and Industrial Research
contact emailpnaicker1@csir.co.za
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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