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PXD032913-1

PXD032913 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphoproteomics reveals rewiring of the insulin signaling network and multi-nodal defects in insulin resistance
DescriptionHere we employed phosphoproteomics to delineate insulin signal transduction in adipocyte cells and adipose tissue. Across a range of insults triggering insulin resistance, we observed marked rewiring of the insulin signaling network. This included both attenuated insulin-responsive phosphorylation, and the emergence of phosphorylation uniquely insulin-regulated in insulin resistance. Identifying dysregulated phosphosites common to multiple insults revealed subnetworks containing novel regulators of insulin action, such as MARK2/3, and causal drivers of insulin resistance. The presence of several GSK3 substrates among these phosphosites led us to establish a pipeline for identifying context-specific kinase substrates. This revealed widespread dysregulation of GSK3 signaling. Pharmacological inhibition of GSK3 partially reversed insulin resistance in cells and tissue explants. These data highlight that insulin resistance is multi-nodal signaling defect that includes dysregulated GSK3 activity.
HostingRepositoryPRIDE
AnnounceDate2023-02-17
AnnouncementXMLSubmission_2023-02-17_09:45:38.029.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSeanHumphrey
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue
InstrumentQ Exactive HF; Orbitrap Exploris 480; Q Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-02 11:42:32ID requested
12023-02-17 09:45:38announced
22023-11-14 08:55:36announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: post-translational modification, adipocyte, mass spectrometry, fat, phosphoproteomics, GSK3, tissue, proteomics, adipose, signal transduction, phosphorylation, insulin signalling,insulin resistance
Contact List
SeanHumphrey
contact affiliationThe University of Sydney, Australia
contact emailsean.j.humphrey@gmail.com
lab head
SeanHumphrey
contact affiliationMurdoch Children's Research Institute
contact emailsean.j.humphrey@gmail.com
dataset submitter
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Dataset FTP location
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