PXD032830-1

PXD032830 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic changes during anoxia and reoxygenation in anoxia-tolerant crucian carp
Description	The freshwater fish crucian carp (Carassius carassius) can survive complete oxygen depletion (anoxia) for several months at low temperatures, achieved by a combination of reduced energy demand and increased glycolysis fueled by large hepatic glycogen stores. In crucian carp, the energy-requiring protein synthesis is controlled in a tissue-specific manner when oxygen levels decrease. During anoxia, translational rates are maintained at almost normoxic levels in brain, while heart and liver translation rates are strongly reduced. However, little is known about how the global proteome of these tissues are affected by oxygen variations. By applying mass spectrometry-based proteomics, 3304 proteins in brain, 3004 proteins in heart and 2516 proteins in liver were detected, of which 66 brain proteins, 243 cardiac proteins and 162 hepatic proteins were differentially expressed during the course of anoxia-reoxygenation compared to normoxic control. The brain proteome showed few differences in response to oxygen variations, indicating that anoxic survival is not regulated through protein expression in this tissue. Cardiac and hepatic adaptions to anoxia included enrichment of mitochondrial proteins involved in aerobic respiration and mitochondrial membrane integrity. We show that enzymes in the electron transport system (ETS) are regulated in a tissue-specific manner since no ETS components were regulated in brain, but were downregulated in heart and upregulated in liver during anoxia and reoxygenation. Furthermore, complement system activation was enriched in heart during anoxia. During reoxygenation, proteins involved in the cristae junction organization were regulated in the heart, possibly explaining how reactive oxygen species can be avoided when oxygen returns in this master of anoxic survival.
HostingRepository	PRIDE
AnnounceDate	2023-01-27
AnnouncementXML	Submission_2023-01-27_12:28:50.032.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032830
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	BerndThiede
SpeciesList	scientific name: Cyprinidae; NCBI TaxID: 7953;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-26 14:28:24	ID requested
⏵ 1	2023-01-27 12:28:50	announced

10.6019/PXD032830;

submitter keyword: crucian carp,Anoxia

AnetteJohansen
contact affiliation	Department of Biosciences, University of Oslo
contact email	anette.johansen@medisin.uio.no
lab head
BerndThiede
contact affiliation	University of Oslo
contact email	bernd.thiede@ibv.uio.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032830
     1. Label: PRIDE project
     2. Name: Quantitative proteomic changes during anoxia and reoxygenation in anoxia-tolerant crucian carp