PXD032386 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A reliable approach for pure Yeast Cell Wall protein isolation from Saccharomyces cerevisiae yeast cells |
Description | Saccharomyces cerevisiae yeast is a fungus presenting a peripheral organelle called the cell wall. The cell wall protects the yeast cell from stress and provides means for communication with the surrounding environment. It has a complex molecular structure, composed of an internal part of cross-linked polysaccharides and an external part of mannoproteins. These latter are very interesting owing to their functional properties, dependent of their molecular features with massive mannosylations. Therefore, the molecular characterization of mannoproteins is a must relying on the optimal isolation and preparation of the cell wall fraction. Multiple methods are well reported for yeast cell wall isolation. The most ap-plied one consists of yeast cell lysis by mechanical disruption. However, applying this classical approach to S288C yeast cells showed a considerable contamination with non-cell wall proteins, mainly comprising mitochondrial proteins. Here-in, we tried to further purify the yeast cell wall preparation by two means: ultracentrifugation and Triton X-100 addition. While the first strategy showed limited outcomes in mitochondrial proteins removal, the second strategy showed optimal results when Triton X-100 was added at 5%, allowing the identification of more mannoproteins and enriching significant-ly their amounts. This promising method could be reliably implemented in lab-scale and industrial processes for “pure” cell wall isolation. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_07:27:36.832.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marie YAMMINE |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-03-18 08:49:07 | ID requested | |
1 | 2022-11-29 03:05:56 | announced | |
⏵ 2 | 2023-11-14 07:27:40 | announced | 2023-11-14: Updated project metadata. |
Publication List
Yammine M, Bray F, Flament S, Picavet A, Lacroix JM, Poilpr, é E, Mouly I, Rolando C, Yeast Cells. ACS Omega, 7(34):29702-29713(2022) [pubmed] |
Keyword List
submitter keyword: Yeast Cell Wall (YCW), ultracentrifugation, Cell Wall Proteins (CWPs), mannoproteins (MNPs), mass spectrometry, bottom-up proteomics, label-free quantification,Saccharomyces cerevisiae |
Contact List
Christian Rolando |
contact affiliation | 1-University of Lille, CNRS, UAR 3290, MSAP, Miniaturisation pour la Synthèse l'Analyse et la Protéomique, F-59650 Villeneuve d’Ascq, France 2-Shrieking sixties, 1-3 Allée Lavoisier, F-59650 Villeneuve d'Ascq, France. |
contact email | christian.rolando@univ-lille.fr |
lab head | |
Marie YAMMINE |
contact affiliation | University of Lille, CNRS, USR 3290, MSAP, Miniaturisation pour la Synthèse, l'Analyse et la Protéomique |
contact email | marie.yammine@univ-lille.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD032386
- Label: PRIDE project
- Name: A reliable approach for pure Yeast Cell Wall protein isolation from Saccharomyces cerevisiae yeast cells