PXD032386

PXD032386 is an original dataset announced via ProteomeXchange.

Title	A reliable approach for pure Yeast Cell Wall protein isolation from Saccharomyces cerevisiae yeast cells
Description	Saccharomyces cerevisiae yeast is a fungus presenting a peripheral organelle called the cell wall. The cell wall protects the yeast cell from stress and provides means for communication with the surrounding environment. It has a complex molecular structure, composed of an internal part of cross-linked polysaccharides and an external part of mannoproteins. These latter are very interesting owing to their functional properties, dependent of their molecular features with massive mannosylations. Therefore, the molecular characterization of mannoproteins is a must relying on the optimal isolation and preparation of the cell wall fraction. Multiple methods are well reported for yeast cell wall isolation. The most ap-plied one consists of yeast cell lysis by mechanical disruption. However, applying this classical approach to S288C yeast cells showed a considerable contamination with non-cell wall proteins, mainly comprising mitochondrial proteins. Here-in, we tried to further purify the yeast cell wall preparation by two means: ultracentrifugation and Triton X-100 addition. While the first strategy showed limited outcomes in mitochondrial proteins removal, the second strategy showed optimal results when Triton X-100 was added at 5%, allowing the identification of more mannoproteins and enriching significant-ly their amounts. This promising method could be reliably implemented in lab-scale and industrial processes for “pure” cell wall isolation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:27:36.832.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marie YAMMINE
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-18 08:49:07	ID requested
1	2022-11-29 03:05:56	announced
⏵ 2	2023-11-14 07:27:40	announced	2023-11-14: Updated project metadata.

Yammine M, Bray F, Flament S, Picavet A, Lacroix JM, Poilpr, é E, Mouly I, Rolando C, Yeast Cells. ACS Omega, 7(34):29702-29713(2022) [pubmed]

submitter keyword: Yeast Cell Wall (YCW), ultracentrifugation, Cell Wall Proteins (CWPs), mannoproteins (MNPs), mass spectrometry, bottom-up proteomics, label-free quantification,Saccharomyces cerevisiae

Christian Rolando
contact affiliation	1-University of Lille, CNRS, UAR 3290, MSAP, Miniaturisation pour la Synthèse l'Analyse et la Protéomique, F-59650 Villeneuve d’Ascq, France 2-Shrieking sixties, 1-3 Allée Lavoisier, F-59650 Villeneuve d'Ascq, France.
contact email	christian.rolando@univ-lille.fr
lab head
Marie YAMMINE
contact affiliation	University of Lille, CNRS, USR 3290, MSAP, Miniaturisation pour la Synthèse, l'Analyse et la Protéomique
contact email	marie.yammine@univ-lille.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032386
     1. Label: PRIDE project
     2. Name: A reliable approach for pure Yeast Cell Wall protein isolation from Saccharomyces cerevisiae yeast cells