PXD032353 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Human iPSCs differentiated into SMCs |
Description | Human iPSCs were differentiated towards an induced-SMC (iSMC) phenotype in a 10-day protocol. Proteomics was performed throughout the entire differentiation time course to provide a robust, well-defined starting and ending cell population. Proteomics data verified iPSC differentiation to iSMCs. Proteomics comparison with primary human SMCs showed a high correlation with iSMCs. After iSMC differentiation, we initiated calcification in the iSMCs by culturing the cells in osteogenic media for 17 days. |
HostingRepository | PRIDE |
AnnounceDate | 2022-10-15 |
AnnouncementXML | Submission_2022-10-14_16:32:18.510.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sasha Singh |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-03-17 04:12:51 | ID requested | |
⏵ 1 | 2022-10-14 16:32:19 | announced | |
2 | 2023-11-14 08:11:34 | announced | 2023-11-14: Updated project metadata. |
Publication List
Atkins SK, Sonawane AR, Brouwhuis R, Barrientos J, Ha A, Rogers M, Tanaka T, Okui T, Kuraoka S, Singh SA, Aikawa M, Aikawa E, Induced pluripotent stem cell-derived smooth muscle cells to study cardiovascular calcification. Front Cardiovasc Med, 9():925777(2022) [pubmed] |
Keyword List
submitter keyword: calcification, smooth muscle cell,Lumos |
Contact List
ElenaAikawa |
contact affiliation | Brigham and Women's Hospital |
contact email | eaikawa@bwh.harvard.edu |
lab head | |
Sasha Singh |
contact affiliation | Brigham and Women's Hospital, Harvard Medical School |
contact email | sasingh@bwh.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD032353 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD032353
- Label: PRIDE project
- Name: Human iPSCs differentiated into SMCs