PXD032332 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment |
Description | Chronic myeloid leukemia (CML) cells circulate between blood and bone marrow niche, representing different microenvironments. We studied the role of the two RNA binding proteins, T-cell-restricted intracellular antigen (TIAR) and the fragile X mental retardation protein (FMRP) in the regulation of protein translation in CML cells residing in settings mimicking peripheral blood microenvironment (PBM) and bone marrow microenvironment (BMM). We studied how conditions shaped the translation process through TIAR and FMRP activity, considering its relevance in therapy resistance. The QuaNCAT mass-spectrometric approach revealed that TIAR and FMRP have a discrete modulatory effect on protein synthesis and thus affect distinct aspects of leukemic cells functioning in the hypoxic niche. We demonstrate that TIAR substantially supports the resistance of CML cells to translation inhibition by homoharringtonine in the BMM. Overall, our results show that targeting post-transcriptional control should be considered when designing anti-leukemia therapeutic solutions. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:46:47.948.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Remigiusz Serwa |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-03-16 09:14:19 | ID requested | |
1 | 2023-05-10 13:38:26 | announced | |
⏵ 2 | 2023-11-14 08:46:55 | announced | 2023-11-14: Updated project metadata. |
Publication List
Wolczyk M, Serwa R, Kominek A, Klejman A, Milek J, Chwa, ł, ek M, Turos-Korgul L, Charzy, ń, ska A, Dabrowski M, Dziembowska M, Skorski T, Piwocka K, Podszywalow-Bartnicka P, TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment. iScience, 26(4):106543(2023) [pubmed] |
Keyword List
submitter keyword: QuaNCAT, FMRP,Chronic myeloid leukemia, TIAR |
Contact List
Dr Paulina Podszywalow-Bartnicka |
contact affiliation | Nencki Institute of Experimental Biology 3 Pasteura Street, 02-093 Warsaw, Poland |
contact email | p.podszywalow@nencki.edu.pl |
lab head | |
Remigiusz Serwa |
contact affiliation | The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences |
contact email | r.serwa@imol.edu.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD032332
- Label: PRIDE project
- Name: TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment