PXD032332

PXD032332 is an original dataset announced via ProteomeXchange.

Title	TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment
Description	Chronic myeloid leukemia (CML) cells circulate between blood and bone marrow niche, representing different microenvironments. We studied the role of the two RNA binding proteins, T-cell-restricted intracellular antigen (TIAR) and the fragile X mental retardation protein (FMRP) in the regulation of protein translation in CML cells residing in settings mimicking peripheral blood microenvironment (PBM) and bone marrow microenvironment (BMM). We studied how conditions shaped the translation process through TIAR and FMRP activity, considering its relevance in therapy resistance. The QuaNCAT mass-spectrometric approach revealed that TIAR and FMRP have a discrete modulatory effect on protein synthesis and thus affect distinct aspects of leukemic cells functioning in the hypoxic niche. We demonstrate that TIAR substantially supports the resistance of CML cells to translation inhibition by homoharringtonine in the BMM. Overall, our results show that targeting post-transcriptional control should be considered when designing anti-leukemia therapeutic solutions.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:47.948.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-16 09:14:19	ID requested
1	2023-05-10 13:38:26	announced
⏵ 2	2023-11-14 08:46:55	announced	2023-11-14: Updated project metadata.

Wolczyk M, Serwa R, Kominek A, Klejman A, Milek J, Chwa, ł, ek M, Turos-Korgul L, Charzy, ń, ska A, Dabrowski M, Dziembowska M, Skorski T, Piwocka K, Podszywalow-Bartnicka P, TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment. iScience, 26(4):106543(2023) [pubmed]

submitter keyword: QuaNCAT, FMRP,Chronic myeloid leukemia, TIAR

Dr Paulina Podszywalow-Bartnicka
contact affiliation	Nencki Institute of Experimental Biology 3 Pasteura Street, 02-093 Warsaw, Poland
contact email	p.podszywalow@nencki.edu.pl
lab head
Remigiusz Serwa
contact affiliation	The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences
contact email	r.serwa@imol.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD032332

PRIDE project URI

• PRIDE
  1. PXD032332
     1. Label: PRIDE project
     2. Name: TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment