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PXD032185-1

PXD032185 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe cohesin acetylation cycle controls chromatin loop length through a PDS5A brake mechanism
DescriptionCohesin structures the genome through the formation of chromatin loops and by holding together the sister chromatids. The acetylation of cohesin’s SMC3 subunit is a dynamic process that involves the acetyltransferase ESCO1 and deacetylase HDAC8. Here we show that this cohesin acetylation cycle controls the 3D genome. ESCO1 restricts the length of chromatin loops and architectural stripes, while HDAC8 promotes the extension of such loops and stripes. This role in controlling loop length turns out to be distinct from the canonical role of cohesin acetylation that protects against WAPL-mediated DNA release. We reveal that acetylation rather controls cohesin’s interaction with PDS5A to restrict chromatin loop length. Our data supports a model in which this PDS5A-bound state acts as a brake that enables the pausing and restart of loop enlargement. The cohesin acetylation cycle hereby provides punctuation in the process of genome folding.
HostingRepositoryPRIDE
AnnounceDate2022-03-14
AnnouncementXMLSubmission_2022-03-14_02:22:13.351.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLiesbeth Hoekman
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-10 11:58:24ID requested
12022-03-14 02:22:13announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Cohesin, ESCO1, HDAC8, PDS5A, PDS5B, WAPL, CTCF, 3D genome, cohesin acetylation cycle, chromatin loops
Contact List
Maarten Altelaar
contact affiliationProteomics Facility, The Netherlands Cancer Institute, Amsterdam, 1066 CX, The Netherlands; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research, Utrecht Institute for Pharmaceutical Sciences, Utrecht University and Netherlands Proteomics Centre, Utrecht, 3584 CH, The Netherlands
contact emailm.altelaar@nki.nl
lab head
Liesbeth Hoekman
contact affiliationThe Netherlands Cancer Institute, Amsterdam, The Netherlands.
contact emaill.hoekman@nki.nl
dataset submitter
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Dataset FTP location
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