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PXD032142-1

PXD032142 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLabel free quantification of LC-MS analysis of Dental pulp cells
DescriptionLC/MS analysis of secretion of dental pulp cell. Methanol/chloroform precipitation was performed for protein purification. Resulting pellets were resolved by MS buffer (8 M urea and 50 mM Tris–HCl pH 8.0), followed by reduction in 5 mM DTT (FUJIFILM WAKO) and alkylation in 27.5 mM iodoacetamide (FUJIFILM WAKO) for 30 min in the dark at room temperature. After being diluted eight times in 50 mM Tris–HCl pH 8.0, proteins were digested overnight with 50 ng Lys-C (FUJIFILM WAKO) and trypsin (Promega, Madison, WI) at 37°C. Peptides were purified using GL-Tips SDB (GL Science, Tokyo, Japan) based on the manufacturer's protocol. The concentration of peptides was measured using a Pierce™ quantitative colorimetric peptide assay kit (Thermo Fisher Scientific). Subsequently, peptides (268 ng each) were injected into an EASY-nLC™ connected to a Q-Exactive PLUSTM (Thermo Fisher Scientific) mass spectrometry system. Protein identification and label-free quantification were performed using Proteome Discoverer 2.2™ (Thermo Fisher Scientific).
HostingRepositoryjPOST
AnnounceDate2023-03-09
AnnouncementXMLSubmission_2023-03-08_07:00:04.361.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAkihito Yamamoto
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-09 01:53:46ID requested
12023-03-08 07:00:04announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Dental pulp, LFQ
Contact List
Akihito Yamamoto
lab head
Akihito Yamamoto
contact affiliationTokushima University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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