PXD032142

PXD032142 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Label free quantification of LC-MS analysis of Dental pulp cells
Description	LC/MS analysis of secretion of dental pulp cell. Methanol/chloroform precipitation was performed for protein purification. Resulting pellets were resolved by MS buffer (8 M urea and 50 mM Tris–HCl pH 8.0), followed by reduction in 5 mM DTT (FUJIFILM WAKO) and alkylation in 27.5 mM iodoacetamide (FUJIFILM WAKO) for 30 min in the dark at room temperature. After being diluted eight times in 50 mM Tris–HCl pH 8.0, proteins were digested overnight with 50 ng Lys-C (FUJIFILM WAKO) and trypsin (Promega, Madison, WI) at 37°C. Peptides were purified using GL-Tips SDB (GL Science, Tokyo, Japan) based on the manufacturer's protocol. The concentration of peptides was measured using a Pierce™ quantitative colorimetric peptide assay kit (Thermo Fisher Scientific). Subsequently, peptides (268 ng each) were injected into an EASY-nLC™ connected to a Q-Exactive PLUSTM (Thermo Fisher Scientific) mass spectrometry system. Protein identification and label-free quantification were performed using Proteome Discoverer 2.2™ (Thermo Fisher Scientific).
HostingRepository	jPOST
AnnounceDate	2023-03-09
AnnouncementXML	Submission_2023-03-08_07:00:04.361.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akihito Yamamoto
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-09 01:53:46	ID requested
⏵ 1	2023-03-08 07:00:04	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Dental pulp, LFQ

submitter keyword: Dental pulp, LFQ

Contact List

Akihito Yamamoto
lab head
Akihito Yamamoto
contact affiliation	Tokushima University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001520/