PXD032098-2

PXD032098 is an original dataset announced via ProteomeXchange.

Title	Structure of the AAA-ATPase Drg1 during extraction of Rlp24 from pre-60S particles
Description	The AAA-ATPase Drg1 is a key factor in eukaryotic ribosome biogenesis that initiates cytoplasmic maturation of the large ribosomal subunit. Drg1 releases the shuttling maturation factor Rlp24 from pre-60S particles shortly after nuclear export, a strict requirement for downstream maturation. The molecular mechanism of release remained elusive. Here, we report a series of cryo-EM structures showing a hand-over-hand translocation mechanism of Drg1 and additional XL-MS data on positioning of Drg1 on pre-60S particles.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:53:32.229.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian Stengel
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-07 03:49:49	ID requested
1	2022-10-14 14:58:12	announced
⏵ 2	2023-11-14 07:53:33	announced	2023-11-14: Updated project metadata.

submitter keyword: large ribosomal subunit,Ribosome biogenesis, AAA-ATPases, substrate recognition, substrate translocation mechanism, single particle cryo-EM

Florian Stengel
contact affiliation	Department of Biology, University of Konstanz, Germany
contact email	florian.stengel@uni-konstanz.de
lab head
Florian Stengel
contact affiliation	Universität Konstanz
contact email	florian.stengel@uni-konstanz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032098
     1. Label: PRIDE project
     2. Name: Structure of the AAA-ATPase Drg1 during extraction of Rlp24 from pre-60S particles