PXD032091

PXD032091 is an original dataset announced via ProteomeXchange.

Title	Proteomic study of epicardial exudate from acute myocardial infarction in rats
Description	The epicardium has a vital role in cardiac development, regeneration, injury, repair, and clinical prognosis, but it is unclear how the epicardium initiates the repair process during an acute myocardial infarction (MI). The objective of this study was to collect epicardial exudate in the early stages of acute MI by optimizing the active hydraulic ventricular through attaching a support system (ASD) and performing a proteomic analysis (proteomics). In order to develop a new pathological method, we need to monitor the real-time molecular changes associated with the epicardium in cardiac diseases and identify new potential therapeutic targets. The results showed the Microporous suction cup-like ASD system as a "second body chamber" that could better collect epicardial exudate from acute MI in rats, and the analysis of differentially expressed proteins (DEPs) in the samples showed that the DEPs within 6 hours of acute MI were mainly involved in the production of precursor metabolites and energy regulation of intracellular proteins, regulation of neuronal projection development, hippocampal signaling pathway, and other processes. In addition, we also found that the levels of acute inflammatory response and complement response in epicardial exudate were decreased, which may be related to the negative regulation of inflammation. In conclusion, the Microporous suction cup-like ASD system provides a new and precise means to study the molecular pathology of cardiovascular diseases (CVD): a collection of samples from the epicardium in situ and their analysis by multiple means in vitro. Through this process, we monitored the progress of the molecular pathology of CVD in real-time, elucidated the regulatory network of epicardial repair, and achieved cardiac repair by regulating the regenerative potential of the epicardium.
HostingRepository	iProX
AnnounceDate	2022-03-05
AnnouncementXML	Submission_2022-03-06_22:29:12.795.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaohui Zhou
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-06 21:09:03	ID requested
1	2022-03-06 21:09:23	announced
⏵ 2	2022-03-06 22:29:13	announced	2022-03-07: Update information.

Dataset with its publication pending

submitter keyword: acute myocardial infarction, epicardial, Microporous suction cup-like ASD system, cardiac repair

Xiaohui Zhou
contact affiliation	China Pharmaceutical University
contact email	zhxh@cpu.edu.cn
lab head
Xiaohui Zhou
contact affiliation	China Pharmaceutical University
contact email	zhxh@cpu.edu.cn
dataset submitter

iProX dataset URI