Update information. The epicardium has a vital role in cardiac development, regeneration, injury, repair, and clinical prognosis, but it is unclear how the epicardium initiates the repair process during an acute myocardial infarction (MI). The objective of this study was to collect epicardial exudate in the early stages of acute MI by optimizing the active hydraulic ventricular through attaching a support system (ASD) and performing a proteomic analysis (proteomics). In order to develop a new pathological method, we need to monitor the real-time molecular changes associated with the epicardium in cardiac diseases and identify new potential therapeutic targets. The results showed the Microporous suction cup-like ASD system as a "second body chamber" that could better collect epicardial exudate from acute MI in rats, and the analysis of differentially expressed proteins (DEPs) in the samples showed that the DEPs within 6 hours of acute MI were mainly involved in the production of precursor metabolites and energy regulation of intracellular proteins, regulation of neuronal projection development, hippocampal signaling pathway, and other processes. In addition, we also found that the levels of acute inflammatory response and complement response in epicardial exudate were decreased, which may be related to the negative regulation of inflammation. In conclusion, the Microporous suction cup-like ASD system provides a new and precise means to study the molecular pathology of cardiovascular diseases (CVD): a collection of samples from the epicardium in situ and their analysis by multiple means in vitro. Through this process, we monitored the progress of the molecular pathology of CVD in real-time, elucidated the regulatory network of epicardial repair, and achieved cardiac repair by regulating the regenerative potential of the epicardium.