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PXD032072-1

PXD032072 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe PripA-TbcrA complex-centered Rab GAP cascade facilitates macropinosome maturation in Dictyostelium
DescriptionMacropinocytosis, an evolutionarily conserved mechanism mediating nonspecific bulk uptake of extracellular fluid, has been ascribed diverse functions. How nascent macropinosomes mature after internalization remains largely unknown. By searching for proteins that localize on macropinosomes during the Rab5-to-Rab7 transition stage in Dictyostelium, we uncover a complex composed of two proteins, which we name PripA and TbcrA. We show that the Rab5-to-Rab7 conversion involves fusion of Rab5-marked early macropinosomes with Rab7-marked late macropinosomes. PripA links the two membrane compartments by interacting with PI(3,4)P2 and Rab7. In addition, PripA recruits TbcrA, which acts as a GAP, to turn off Rab5. Thus, the conversion to Rab7 is linked to inactivation of the upstream Rab5. Consistently, disruption of either pripA or tbcrA impairs Rab5 inactivation and macropinocytic cargo processing. Therefore, the PripA-TbcrA complex is the central component of a Rab GAP cascade that facilitates programmed Rab switch and efficient cargo trafficking during macropinosome maturation.
HostingRepositoryPRIDE
AnnounceDate2022-03-14
AnnouncementXMLSubmission_2022-03-14_02:41:42.541.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHui Tu
SpeciesList scientific name: Dictyostelium discoideum (Slime mold); NCBI TaxID: 44689;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-05 05:20:26ID requested
12022-03-14 02:41:42announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: macropinocytosis, PripA, TbcrA, Rab5, Rab7
Contact List
Huaqing Cai
contact affiliationInstitute of Biophysics, Chinese Academy of Sciences
contact emailhuaqingcai@ibp.ac.cn
lab head
Hui Tu
contact affiliationInsititute of Biophysics
contact email18070285210@163.com
dataset submitter
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Dataset FTP location
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