PXD032062-1

PXD032062 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Multiplexed Quantitative Cell Surface Proteomics by Combination of TMT Labeling and Biotinylated Peptide Enrichment
Description	We examined changes in surface proteome of HeLa cells stimulated with epidermal growth factor (EGF), which is well known to induce the internalization and degradation of EGF receptor (EGFR). Surface biotinylation using Sulfo-NHS-biotin was performed at four different times after EGF stimulation in multiple biological replicates, followed by digestion of cellular proteins with trypsin. After TMT labeling and mixing of digested peptides, biotinylated peptides were enriched using Tamavidin 2-REV, followed by LC-MS/MS analysis.
HostingRepository	jPOST
AnnounceDate	2023-03-04
AnnouncementXML	Submission_2023-03-03_07:00:05.337.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kohei Nishino
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; N6-biotinyl-L-lysine; TMT6plex reporter+balance reagent acylated N-terminal; TMT6plex reporter+balance reagent N-acylated residue; L-methionine sulfoxide
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-04 00:28:04	ID requested
⏵ 1	2023-03-03 07:00:05	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Biotinylated Peptides, Tamavidin 2-REV, cell surface proteome, TMT

submitter keyword: Biotinylated Peptides, Tamavidin 2-REV, cell surface proteome, TMT

Contact List

Hidetaka Kosako
lab head
Kohei Nishino
contact affiliation	Tokushima University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001515/