PXD031976 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative chemoproteomic identification of lysine carboxylation in proteins |
Description | Carbon dioxide is an abundant biological gas that drives adaptive physiological responses within organisms from all domains. The basic biochemical mechanisms by which proteins serve as sensors of CO2 are, therefore, of great interest. CO2 is a potent electrophilic, and thus one way it can modulate protein biochemistry is by carboxylation of the primary amines group of lysines. However, the resulting cabamates spontaneously decompose, giving off CO2, which makes studying this modification notoriously challenging. Here we describe a chemoproteomic method to stably mimic CO2-carboxylated lysine residues in proteins by reacting them with OCNH. We leverage this method to develop a competition based strategy to quantitatively identify CO2-carboxylatedlysines of proteins and use it to explore the lysine ‘carboxylome’. |
HostingRepository | PRIDE |
AnnounceDate | 2023-09-09 |
AnnouncementXML | Submission_2023-09-09_14:46:29.079.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | DustinKing |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Synechocystis sp.; NCBI TaxID: 1143; |
ModificationList | carbamoylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-03-01 10:31:19 | ID requested | |
⏵ 1 | 2023-09-09 14:46:30 | announced | |
2 | 2023-11-14 09:05:23 | announced | 2023-11-14: Updated project metadata. |
Publication List
King DT, Zhu S, Hardie DB, Serrano-Negr, ó, n JE, Madden Z, Kolappan S, Vocadlo DJ, -dependent lysine carboxylation in proteins. Nat Chem Biol, 18(7):782-791(2022) [pubmed] |
Keyword List
submitter keyword: CO2, lysine carboxylation, chemoproteomics, homocitrulline |
Contact List
DavidVocadlo |
contact affiliation | Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada Department of Chemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada |
contact email | dvocadlo@sfu.ca |
lab head | |
DustinKing |
contact affiliation | Simon Fraser University |
contact email | dustink@sfu.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031976
- Label: PRIDE project
- Name: Quantitative chemoproteomic identification of lysine carboxylation in proteins