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PXD031862-2

PXD031862 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleExtracellular vesicles derived from the choroid plexus trigger neural stem cells differentiation
DescriptionAn organized network of beating cilia transports cerebrospinal fluid (CSF) through the ventricles of the brain. CSF takes along diverse solutes including extracellular vesicles (EV), which arise from the choroid plexus, a secretory epithelium that reaches into the ventricles. Along their path through the ventricles, EVs have the opportunity to contact neural stem cell (NSC) niches. We purified EVZ310 produced by a choroid plexus-cell line, by differential centrifugation and flotation in iodixanol density gradients. We co-cultured suspensions of EVZ310 with NSC from the lateral and third ventricle. Alternatively, EVZ310 were dried-down on a polymer substrate and a suspension of NSCs was added. In both cases, EV Z310 induced, in a dose dependent manner, the NSC to rapidly form cellular networks accompanied by the expression of genes characteristic for neurons (Tuj1) and astrocytes (Gfap). NSCs from either origin responded to EVZ310 qualitatively and quantitatively in a very similar way. By contrast, EVMEF purified from mouse embryonic fibroblasts had little effect on both types of NSC. Mass spectrometry revealed significant differences in composition between EVMEF and EVZ310 proteomes. Notably, EVZ310 were enriched for the membrane or membrane associated proteins known to be involved in neurogenesis, cell differentiation, membrane trafficking and membrane organization.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:55:06.560.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIvan Silbern
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF-X; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-24 00:57:57ID requested
12022-12-18 10:28:56announced
22023-11-14 08:55:07announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: cerebrospinal fluid, neural stem cells, mass spectrometry,Extracellular vesicles, exosome assay, neural stem cell differentiation, choroid plexus derived vesicle
Contact List
Prof. Dr. Henning Urlaub
contact affiliationBioanalytical Mass Spectrometry Group, Max-Planck-Institute for Multidisciplinary Sciences, Göttingen, Germany; Institute for Clinical Chemistry, University Medical Center Göttingen, Göttingen, Germany
contact emailhenning.urlaub@mpinat.mpg.de
lab head
Ivan Silbern
contact affiliationMPI Biophysical Chemistry
contact emailivansilbern@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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