PXD031784-2

PXD031784 is an original dataset announced via ProteomeXchange.

Title	Proteomic and functional characterization of intra-tumor heterogeneity in human endometrial cancer.
Description	Endometrial cancer (EC) is one of the most frequently diagnosed gynecological cancers worldwide, and its prevalence has increased by more than 50% over the last two decades. Despite the understanding of the major signaling pathways driving the growth and metastasis of EC cells, clinical trials targeting these signaling pathways in human patients have reported poor outcomes. Heterogeneous nature of EC is suspected to be one of the key reasons for the failure of targeted therapies. However, no study so far has explored EC heterogeneity within the same patient at the proteomic level. In this study, we isolated proteins from tumor tissue samples obtained from different sites of EC from individual patients (~2-4 samples/patient). We then performed a SWATH-based comparative proteomic analysis, using liquid chromatography-tandem mass spectrometry (LC-MS/MS), to profile the protein content of different areas within EC tissue. Our results highlighted an average of 1424 unique proteins in 20 patient-derived EC tissues with a confidence corresponding to a false discovery rate below 1%. We have identified protein biomarkers that differentiate between premenopausal vs postmenopausal cancer, macroscopic vs microscopic tumor, and more vs less invasive cancer, and DNA mismatch repair defective vs intact tumor. Furthermore, the data revealed a list of unique proteins that, for the same patient, exist only in a single EC location but are not present in other locations within the same tumor. Overall, our proteomic analysis highlighted that tumor tissue samples collected from different sites of EC within the same patient can harbor diverse protein profiles. Importantly, this study sets the foundation for further investigations into the mechanisms of endometrial heterogeneity and some of the proteins identified here may represent potential novel EC drug targets.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:32:04.779.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031784
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Muhammad Fairuz Jamaluddin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-21 03:09:16	ID requested
1	2022-10-14 02:07:50	announced
⏵ 2	2023-11-14 08:32:08	announced	2023-11-14: Updated project metadata.

Jamaluddin MFB, Ko YA, Ghosh A, Syed SM, Ius Y, O'Sullivan R, Netherton JK, Baker MA, Nahar P, Jaaback K, Tanwar PS, Proteomic and functional characterization of intra-tumor heterogeneity in human endometrial cancer. Cell Rep Med, 3(9):100738(2022) [pubmed]

10.6019/PXD031784;

ProteomeXchange project tag: Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: Endometrial Cancer

Pradeep Tanwar
contact affiliation	School of Biomedical Sciences, The University of Newcastle
contact email	pradeep.tanwar@newcastle.edu.au
lab head
Muhammad Fairuz Jamaluddin
contact affiliation	The University of Newcastle
contact email	muhammad.jamaluddin@newcastle.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD031784

PRIDE project URI

• PRIDE
  1. PXD031784
     1. Label: PRIDE project
     2. Name: Proteomic and functional characterization of intra-tumor heterogeneity in human endometrial cancer.