PXD031639

PXD031639 is an original dataset announced via ProteomeXchange.

Title	The Proteome and Transcriptome of Stress Granules and P-Bodies during Human T Lymphocytes Activation
Description	Stress granules (SGs) and processing bodies (PBs) are membraneless cytoplasmic assemblies regulating mRNAs under environmental stress such as viral infections, neurological disorders, or cancer. Upon antigen stimulation, T lymphocytes mediate their immune functions under regulatory mechanisms involving SGs and PBs. However, the impact of T cell activation on such complexes, in term of formation, constitution and relationship remains unknown. Here, by combining proteomic, transcriptomic and immunofluorescence approaches, we simultaneously characterized the SGs and PBs from primary human T lymphocytes pre- and post-stimulation. The proteomes and transcriptomes of SGs and PBs were identified, unveiling an unanticipated molecular and functional complementarity. Notwithstanding, these granules keep distinct spatial organizations and abilities to interact with mRNAs. This comprehensive characterization of the RNP granule proteomic and transcriptomic landscapes provides a unique resource for future investigations on SGs and PBs in T lymphocytes.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:40.117.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Don-Marc Franchini
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-13 12:59:09	ID requested
1	2023-03-10 11:25:06	announced
⏵ 2	2023-11-14 08:55:40	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: human T lymphocytes,ribonucleoprotein granules, proteome

Camille Laurent
contact affiliation	Cancer Research Center of Toulouse (CRCT) 2 Avenue Hubert Curien 31037 Toulouse
contact email	laurent.c@chu-toulouse.fr
lab head
Don-Marc Franchini
contact affiliation	Cancer Research Center of Toulouse (CRCT)
contact email	don-marc.franchini@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD031639

PRIDE project URI

• PRIDE
  1. PXD031639
     1. Label: PRIDE project
     2. Name: The Proteome and Transcriptome of Stress Granules and P-Bodies during Human T Lymphocytes Activation