PXD031540-1

PXD031540 is an original dataset announced via ProteomeXchange.

Title	Indentification of RecBCD Auxiliary ATP binding
Description	The RecBCD helicase initiates double-stranded break repair in bacteria by processively unwinding DNA with a rate approaching ~1,600 bp·second, but the mechanism enabling such a fast rate is unknown. By using crosslinking followed by mass spectrometry, we revealed evidence for the existence of auxiliary binding sites in the RecC subunit. This was validated by equilibrium and time-resolved binding experiments, ensemble, and single-molecule unwinding assays. The essentiality and functionality of these sites are further demonstrated by their impact on the survival of E.coli after exposure to damage-inducing radiation.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-20_09:10:20.718.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oded Kleifeld
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-09 07:48:53	ID requested
⏵ 1	2022-05-20 09:10:21	announced

Zananiri R, Mangapuram Venkata S, Gaydar V, Yahalom D, Malik O, Rudnizky S, Kleifeld O, Kaplan A, Henn A, Auxiliary ATP binding sites support DNA unwinding by RecBCD. Nat Commun, 13(1):1806(2022) [pubmed]

submitter keyword: RecBCD, AzidoATP, AzidoAdenosine

Arnon Henn
contact affiliation	Associate Professor Faculty of Biology Technion-Israel Institute of Technology, Technion City, Haifa 3200003, Israel
contact email	arnon.henn@technion.ac.il
lab head
Oded Kleifeld
contact affiliation	Technion
contact email	okleifeld@technion.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD031540

PRIDE project URI

• PRIDE
  1. PXD031540
     1. Label: PRIDE project
     2. Name: Indentification of RecBCD Auxiliary ATP binding