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PXD031540 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIndentification of RecBCD Auxiliary ATP binding
DescriptionThe RecBCD helicase initiates double-stranded break repair in bacteria by processively unwinding DNA with a rate approaching ~1,600 bp·second, but the mechanism enabling such a fast rate is unknown. By using crosslinking followed by mass spectrometry, we revealed evidence for the existence of auxiliary binding sites in the RecC subunit. This was validated by equilibrium and time-resolved binding experiments, ensemble, and single-molecule unwinding assays. The essentiality and functionality of these sites are further demonstrated by their impact on the survival of E.coli after exposure to damage-inducing radiation.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterOded Kleifeld
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-09 07:48:53ID requested
12022-05-20 09:10:21announced
Publication List
Zananiri R, Mangapuram Venkata S, Gaydar V, Yahalom D, Malik O, Rudnizky S, Kleifeld O, Kaplan A, Henn A, Auxiliary ATP binding sites support DNA unwinding by RecBCD. Nat Commun, 13(1):1806(2022) [pubmed]
Keyword List
submitter keyword: RecBCD, AzidoATP, AzidoAdenosine
Contact List
Arnon Henn
contact affiliationAssociate Professor Faculty of Biology Technion-Israel Institute of Technology, Technion City, Haifa 3200003, Israel
contact emailarnon.henn@technion.ac.il
lab head
Oded Kleifeld
contact affiliationTechnion
contact emailokleifeld@technion.ac.il
dataset submitter
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Dataset FTP location
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