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PXD031490-1

PXD031490 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCyclodepsipeptides, solonamides, influence motility in the native producer Photobacterium galatheae S2753
DescriptionThe marine bacterium Photobacterium galatheae S2753 produces a group of cyclodepsipeptides, solonamides, which impede the virulence but not the survival of Staphylococcus aureus. Besides the invaluable antivirulence activity, little is known about the biosynthesis and physiological function of solonamides in the native producer. This study generated a solonamides-deficient mutant, Δsol, by in-frame deleting the sol gene, thereby identifying the core gene for solonamides biosynthesis. By annotation from antiSMASH, the biosynthetic pathway of solonamides in S2753 was also proposed. Mass spectrometry analysis of cell extracts found that deficiency of solonamides production influenced the production of several novel lipids, but not the overall secondary metabolite profile. Physiological comparison between Δsol and wild-type S2753 demonstrated that growth dynamics and biofilm formation of both strains were similar; however, Δsol displayed reduced swimming rings as compared to the wild-type. The swimming ring size of Δsol was restored to the level of wild-type strain by adding solonamide B, indicating that solonamide B influences regulate the swimming behavior of P. galatheae S2753. Proteomic analysis of the Δsol and wild-type found that eliminating solonamides influenced many cellular processes, including swimming-related proteins and that solonamides trigger the motility of S2753 likely by adjusting the cellular cyclic di-GMP concentration. In conclusion, our results revealed the biosynthetic pathway of solonamides and their ecological benefits to P. galatheae S2753 by enhancing the swimming velocity, likely by altering the motile physiology.
HostingRepositoryPRIDE
AnnounceDate2022-08-17
AnnouncementXMLSubmission_2022-08-17_12:08:59.166.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterShengda Zhang
SpeciesList scientific name: Photobacterium galatheae; NCBI TaxID: 1654360;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-07 08:25:44ID requested
12022-08-17 12:08:59announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Solonamides, Photobacterium galatheae,LC-MS, proteome, secretome
Contact List
Lone Gram
contact affiliationDepartment of Bioengineering, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark;
contact emailgram@bio.dtu.dk
lab head
Shengda Zhang
contact affiliationDTU Bioengineering
contact emailshez@dtu.dk
dataset submitter
Full Dataset Link List
Dataset FTP location
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