PXD031488 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Profiling the anandamide reuptake inhibitor WOBE437 |
Description | Anandamide, or N-arachidonoylethanolamine (AEA), is a signaling lipid that modulates neurotransmitter release via activation of the cannabinoid receptor type 1 (CB1R) in the brain. Termination of anandamide signaling is thought to be mediated via a facilitated cellular reuptake process utilizing an unknown transporter protein. Recently, WOBE437 has been reported as a novel, natural product-based inhibitor of AEA reuptake that is active in cellular and in vivo models. To profile its target interaction landscape, we synthesized pac-WOBE, a photoactivatable probe derivative of WOBE437, and performed chemical proteomics in mouse neuroblastoma Neuro-2a cells. Surprisingly, WOBE437, but not OMDM-1, was found to increase AEA uptake in Neuro-2a cells. In line, WOBE437 reduced cellular levels of AEA and related N-acylethanolamines (NAEs). Using pac-WOBE, we identified saccharopine dehydrogenase-like oxidoreductase (SCCPDH), vesicle amine transport 1 (VAT1) and ferrochelatase (FECH) as WOBE437-interacting proteins in Neuro-2a cells |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:55:06.331.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Berend Gagestein |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-02-07 08:19:28 | ID requested | |
1 | 2022-05-04 04:22:58 | announced | |
⏵ 2 | 2023-11-14 08:55:07 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: ABPP, photoaffinity,Proteomics, endocannabinoid |
Contact List
Mario van der Stelt |
contact affiliation | Professor of Molecular physiology Leiden Institute of Chemistry LIC/Chemical Biology Leiden University The Netherlands |
contact email | m.van.der.stelt@chem.leidenuniv.nl |
lab head | |
Berend Gagestein |
contact affiliation | Leiden University |
contact email | b.gagestein@lic.leidenuniv.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031488
- Label: PRIDE project
- Name: Profiling the anandamide reuptake inhibitor WOBE437