PXD031488

PXD031488 is an original dataset announced via ProteomeXchange.

Title	Profiling the anandamide reuptake inhibitor WOBE437
Description	Anandamide, or N-arachidonoylethanolamine (AEA), is a signaling lipid that modulates neurotransmitter release via activation of the cannabinoid receptor type 1 (CB1R) in the brain. Termination of anandamide signaling is thought to be mediated via a facilitated cellular reuptake process utilizing an unknown transporter protein. Recently, WOBE437 has been reported as a novel, natural product-based inhibitor of AEA reuptake that is active in cellular and in vivo models. To profile its target interaction landscape, we synthesized pac-WOBE, a photoactivatable probe derivative of WOBE437, and performed chemical proteomics in mouse neuroblastoma Neuro-2a cells. Surprisingly, WOBE437, but not OMDM-1, was found to increase AEA uptake in Neuro-2a cells. In line, WOBE437 reduced cellular levels of AEA and related N-acylethanolamines (NAEs). Using pac-WOBE, we identified saccharopine dehydrogenase-like oxidoreductase (SCCPDH), vesicle amine transport 1 (VAT1) and ferrochelatase (FECH) as WOBE437-interacting proteins in Neuro-2a cells
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:06.331.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Berend Gagestein
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-07 08:19:28	ID requested
1	2022-05-04 04:22:58	announced
⏵ 2	2023-11-14 08:55:07	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: ABPP, photoaffinity,Proteomics, endocannabinoid

Mario van der Stelt
contact affiliation	Professor of Molecular physiology Leiden Institute of Chemistry LIC/Chemical Biology Leiden University The Netherlands
contact email	m.van.der.stelt@chem.leidenuniv.nl
lab head
Berend Gagestein
contact affiliation	Leiden University
contact email	b.gagestein@lic.leidenuniv.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD031488

PRIDE project URI

• PRIDE
  1. PXD031488
     1. Label: PRIDE project
     2. Name: Profiling the anandamide reuptake inhibitor WOBE437