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PXD031461-1

PXD031461 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative proteomics of airway epithelial cells from COPD and control pre- and post-influenza virus infection
DescriptionChronic obstructive pulmonary disease (COPD) collectively refers to chronic and progressive lung diseases causing not fully reversible limitations in airflow. COPD patients are at high risk for severe respiratory symptoms upon influenza virus infection. Airway epithelial cells provide the first-line antiviral defense, but whether their susceptibility and response to influenza virus infection changes in COPD has not been elucidated. Therefore, this study aimed to i) compare the susceptibility of COPD- and control-derived airway epithelium to influenza virus, and ii) assess protein changes during influenza virus infection by quantitative proteomics. Fluorescent stainings confirmed expression of human- and avian-type influenza virus receptors in primary human bronchial epithelial cells (phBECs) from COPD patients (n=4) and controls (n=3) differentiated at the air-liquid interface. Subjects were closely matched in age, sex, and smoking history and, for COPD-derived phBECs, included stage II (n=2), stage III (n=1) and stage IV (n=1). Proteomics of fully differentiated phBECs pre- and post-influenza A virus infection with A/Puerto Rico/8/34 (PR8) revealed no significant differences between GOLD stage II/ III COPD (n=3) and control phBECs in terms of flu receptor expression, cell type composition, virus replication, or protein profile pre- and post-infection. In contrast, COPD GOLD stage IV phBECs (n=1) showed a distinct pattern of flu receptor expression and a typical COPD phenotype as assessed by a literature-derived panel of 20 proteins and quantification of cell type composition. Independent of health state, proteomics showed a robust antiviral response to influenza virus infection, as well as upregulation of several novel influenza virus-regulated proteins.
HostingRepositoryPRIDE
AnnounceDate2022-12-29
AnnouncementXMLSubmission_2022-12-29_11:32:35.629.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJulianeMerl-Pham
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-05 13:47:07ID requested
12022-12-29 11:32:36announced
22023-11-14 08:55:07announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: Chronic obstructive pulmonary disease (COPD), primary human bronchial epithelial cells, differentiated, proteomics, organotypic, influenza virus, flu receptor, airlift
Contact List
ClaudiaStaab-Weijnitz
contact affiliationInstitute of Lung Health and Immunity and Comprehensive Pneumology Center with the CPC-M BioArchive, Helmholtz Zentrum München, Member of the German Center of Lung Research (DZL) - München (Germany)
contact emailstaab-weijnitz@helmholtz-muenchen.de
lab head
JulianeMerl-Pham
contact affiliationResearch Unit Protein Science, Helmholtz Center Munich
contact emailjuliane.merl@helmholtz-muenchen.de
dataset submitter
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Dataset FTP location
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