PXD031440 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Establishment and characterization of a tumoroid biobank derived from dog patients’ mammary tumors for translational research |
Description | Breast cancer is the most frequent cancer among women causing the greatest number of cancer-related deaths. Cancer heterogeneity is a main obstacle to therapies. Around 96% of the drugs fail from discovery to the clinical trial phase probably because of the current unreliable preclinical models. New models emerge such as companion dogs who develop spontaneous mammary tumors resembling human breast cancer in many clinical and molecular aspects. The present work aimed at developing a robust canine mammary tumor model in the form of tumoroids which recapitulate the tumor diversity and heterogeneity. We conducted a complete characterization of these canine mammary tumoroids through histologic, molecular and proteomic analysis, demonstrating their strong similarity to the primary tumor. We demonstrated that these tumoroids can be used as a drug screening model. Due to easy tissue availability, tumoroids can be produced at larger scale and cryopreserved to constitute a biobank. We have demonstrated that cryopreserved tumoroids keep the same histologic and molecular features (ER, PR and HER2 expression) as fresh tumoroids. Two techniques of cryopreservation were compared demonstrating that tumoroids made from frozen tumor material allowed to maintain a higher molecular diversity. These findings revealed that canine mammary tumoroids can be easily generated at large scale and can represent a more reliable preclinical model to investigate tumorigenesis mechanisms and develop new treatments for both veterinary and human medicine. |
HostingRepository | PRIDE |
AnnounceDate | 2023-03-11 |
AnnouncementXML | Submission_2023-03-11_02:27:13.471.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | AntonellaRaffo Romero |
SpeciesList | scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-02-04 15:23:51 | ID requested | |
⏵ 1 | 2023-03-11 02:27:14 | announced | |
2 | 2023-11-14 08:40:43 | announced | 2023-11-14: Updated project metadata. |
Publication List
Raffo-Romero A, Aboulouard S, Bouchaert E, Rybicka A, Tierny D, Hajjaji N, Fournier I, Salzet M, Duhamel M, Establishment and characterization of canine mammary tumoroids for translational research. BMC Biol, 21(1):23(2023) [pubmed] |
Keyword List
submitter keyword: dog patients, drug screening,breast cancer, tumoroid, biobank |
Contact List
MichelSalzet |
contact affiliation | PRISM - Unite Inserm 1192 Universite de Lille France |
contact email | michel.salzet@univ-lille.fr |
lab head | |
AntonellaRaffo Romero |
contact affiliation | PRISM Laboratory - Inserm U1192 Univerty of Lille |
contact email | antonella.raffo-romero@univ-lille.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031440
- Label: PRIDE project
- Name: Establishment and characterization of a tumoroid biobank derived from dog patients’ mammary tumors for translational research