PXD031440

PXD031440 is an original dataset announced via ProteomeXchange.

Title	Establishment and characterization of a tumoroid biobank derived from dog patients’ mammary tumors for translational research
Description	Breast cancer is the most frequent cancer among women causing the greatest number of cancer-related deaths. Cancer heterogeneity is a main obstacle to therapies. Around 96% of the drugs fail from discovery to the clinical trial phase probably because of the current unreliable preclinical models. New models emerge such as companion dogs who develop spontaneous mammary tumors resembling human breast cancer in many clinical and molecular aspects. The present work aimed at developing a robust canine mammary tumor model in the form of tumoroids which recapitulate the tumor diversity and heterogeneity. We conducted a complete characterization of these canine mammary tumoroids through histologic, molecular and proteomic analysis, demonstrating their strong similarity to the primary tumor. We demonstrated that these tumoroids can be used as a drug screening model. Due to easy tissue availability, tumoroids can be produced at larger scale and cryopreserved to constitute a biobank. We have demonstrated that cryopreserved tumoroids keep the same histologic and molecular features (ER, PR and HER2 expression) as fresh tumoroids. Two techniques of cryopreservation were compared demonstrating that tumoroids made from frozen tumor material allowed to maintain a higher molecular diversity. These findings revealed that canine mammary tumoroids can be easily generated at large scale and can represent a more reliable preclinical model to investigate tumorigenesis mechanisms and develop new treatments for both veterinary and human medicine.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:40:42.810.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antonella Raffo Romero
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-04 15:23:51	ID requested
1	2023-03-11 02:27:14	announced
⏵ 2	2023-11-14 08:40:43	announced	2023-11-14: Updated project metadata.

Raffo-Romero A, Aboulouard S, Bouchaert E, Rybicka A, Tierny D, Hajjaji N, Fournier I, Salzet M, Duhamel M, Establishment and characterization of canine mammary tumoroids for translational research. BMC Biol, 21(1):23(2023) [pubmed]

submitter keyword: dog patients, drug screening,breast cancer, tumoroid, biobank

Michel Salzet
contact affiliation	PRISM - Unite Inserm 1192 Universite de Lille France
contact email	michel.salzet@univ-lille.fr
lab head
Antonella Raffo Romero
contact affiliation	PRISM Laboratory - Inserm U1192 Univerty of Lille
contact email	antonella.raffo-romero@univ-lille.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD031440

PRIDE project URI

• PRIDE
  1. PXD031440
     1. Label: PRIDE project
     2. Name: Establishment and characterization of a tumoroid biobank derived from dog patients’ mammary tumors for translational research