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PXD031433

PXD031433 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphoproteomic analysis of isolated B-Cells from AKT overexpression mice compared to wild type mice after stimulation
DescriptionProject description Isolated B-Cells pellets of 16 mice (WT and AKT overexpression mice +/- stimulation, four biological replicates each) were prepared for whole proteome and phosphoproteome analysis. Sample processing protocol Cell pellets were lyzed in a Urea based lysis buffer by sonication in the Bioruptor (Diagenode) device. The resulting proteins were digested using the FASP protocol, followed by phosphopeptides enrichment of 200 µg peptide amount with Zr-IMAC HP magnetic beads (ReSyn Biosciences). All samples were measured in triplicates on nanoElute and timsTOF Pro for proteome samples and timsTOF SCP for phosphoproteome samples (Bruker) both operated in data-independent acquisition mode (DIA). Data processing protocol The DIA rawfiles were processed using DIA-NN v1.8 in library free mode. The built-in library prediction from a mouse FASTA database from uniport.org was utilized. Data analysis and statistical testing was performed using R Scripts available at the Github repositories https://github.com/thmichna.
HostingRepositoryjPOST
AnnounceDate2023-02-04
AnnouncementXMLSubmission_2023-02-03_07:00:05.238.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterThomas Michna
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListS-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide
InstrumenttimsTOF SCP; timsTOF Pro 2
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-04 02:02:36ID requested
12023-02-03 07:00:05announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: phosphoproteomics, B-cells, mouse, LC-MS/MS, Zr-IMAC phosphoenrichment
Contact List
Stefan Tenzer
lab head
Thomas Michna
contact affiliationUniversity Medical Center Mainz
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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