PXD031433
PXD031433 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteomic analysis of isolated B-Cells from AKT overexpression mice compared to wild type mice after stimulation |
Description | Project description Isolated B-Cells pellets of 16 mice (WT and AKT overexpression mice +/- stimulation, four biological replicates each) were prepared for whole proteome and phosphoproteome analysis. Sample processing protocol Cell pellets were lyzed in a Urea based lysis buffer by sonication in the Bioruptor (Diagenode) device. The resulting proteins were digested using the FASP protocol, followed by phosphopeptides enrichment of 200 µg peptide amount with Zr-IMAC HP magnetic beads (ReSyn Biosciences). All samples were measured in triplicates on nanoElute and timsTOF Pro for proteome samples and timsTOF SCP for phosphoproteome samples (Bruker) both operated in data-independent acquisition mode (DIA). Data processing protocol The DIA rawfiles were processed using DIA-NN v1.8 in library free mode. The built-in library prediction from a mouse FASTA database from uniport.org was utilized. Data analysis and statistical testing was performed using R Scripts available at the Github repositories https://github.com/thmichna. |
HostingRepository | jPOST |
AnnounceDate | 2023-02-04 |
AnnouncementXML | Submission_2023-02-03_07:00:05.238.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Thomas Michna |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | S-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide |
Instrument | timsTOF SCP; timsTOF Pro 2 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-02-04 02:02:36 | ID requested | |
⏵ 1 | 2023-02-03 07:00:05 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: phosphoproteomics, B-cells, mouse, LC-MS/MS, Zr-IMAC phosphoenrichment |
Contact List
Stefan Tenzer | |
---|---|
lab head | |
Thomas Michna | |
contact affiliation | University Medical Center Mainz |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001469/ |