Project description Isolated B-Cells pellets of 16 mice (WT and AKT overexpression mice +/- stimulation, four biological replicates each) were prepared for whole proteome and phosphoproteome analysis. Sample processing protocol Cell pellets were lyzed in a Urea based lysis buffer by sonication in the Bioruptor (Diagenode) device. The resulting proteins were digested using the FASP protocol, followed by phosphopeptides enrichment of 200 µg peptide amount with Zr-IMAC HP magnetic beads (ReSyn Biosciences). All samples were measured in triplicates on nanoElute and timsTOF Pro for proteome samples and timsTOF SCP for phosphoproteome samples (Bruker) both operated in data-independent acquisition mode (DIA). Data processing protocol The DIA rawfiles were processed using DIA-NN v1.8 in library free mode. The built-in library prediction from a mouse FASTA database from uniport.org was utilized. Data analysis and statistical testing was performed using R Scripts available at the Github repositories https://github.com/thmichna.