PXD031403

PXD031403 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis underlying the impact of early life nutrition on the molecular control of testicular development in the young bull calf.
Description	It has been established that enhanced early life nutrition progresses sexual development in the bull calf through neuroendocrine signalling via the hypothalamic-pituitary-testicular axis. However, the underlying molecular mechanisms regulating this process have not been fully elucidated. This study measured the impact of contrasting feeding regimes in the first 12 wk of life, known to impact age at puberty, on the proteomic landscape of the testes of bull calves. Holstein bull calves with a mean (±SD) bodyweight and age of 48.8 (± 5.3) kg and 17.5 (± 2.8) days, were designated to high (HI; n=10) or moderate (MOD; n=10) dietary groups, with diets designed to provoke growth rates of 1.0 and 0.5 kg/day, respectively. At 12 wk of age, all calves were euthanized, and testes parenchyma harvested. HI calves were heavier at slaughter (112.4 v 88.7 (2.98) kg, P<0.001), and had a greater average daily gain (ADG) of (0.88 v 0.58 kg, P<0.001). The turquoise network from the protein analyses contained the protein CDH13 which is involved in testes development. Gene ontology analysis of the turquoise network revealed enrichment of genes with functions related to cholesterol biosynthesis, IGF-1 signalling, insulin receptor/secretion signalling, androgen signalling and Sertoli cell junction signalling.
HostingRepository	PRIDE
AnnounceDate	2022-08-30
AnnouncementXML	Submission_2022-08-30_02:00:42.412.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephen Coen
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-03 03:41:39	ID requested
⏵ 1	2022-08-30 02:00:42	announced

Dataset with its publication pending

submitter keyword: Nutrition, testes, protein, bull calf, early life

David Kenny
contact affiliation	Animal Bioscience Department, Teagasc (lab head)
contact email	david.kenny@teagasc.ie
lab head
Stephen Coen
contact affiliation	Teagasc
contact email	scoen101@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031403
     1. Label: PRIDE project
     2. Name: Proteomic analysis underlying the impact of early life nutrition on the molecular control of testicular development in the young bull calf.