PXD031381-1

PXD031381 is an original dataset announced via ProteomeXchange.

Title	Structural requirements for photo-induced RNA-protein cross-linking
Description	Cross-linking of isotope-labelled RNA coupled with mass spectrometry (CLIR-MS) was used to study the UV cross-linking behavior of in vitro reconstituted FOX1 RRM in complex with its cognate RNA sequence, the Fox binding element (FBE), (U)GCAUGU. The FBE heptanucleotide was subsequently mutated, and the impact on UV cross-linking and affinity investigated. Cross-linking was performed using irradiation under 254 nm light, relying on the inherent reactivity of ribonucleotides.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_08:34:32.945.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christopher Sarnowski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-01 03:41:56	ID requested
⏵ 1	2022-06-09 08:34:33	announced

Kn, ö, rlein A, Sarnowski CP, de Vries T, Stoltz M, G, ö, tze M, Aebersold R, Allain FH, Leitner A, Hall J, -stacking interactions initiate photo cross-linking in RNA-protein complexes. Nat Commun, 13(1):2719(2022) [pubmed]

submitter keyword: UV cross-linking

MBNL1

FOX1

PTBP1

SF3A1

U1

U2

cross-linking

XL-MS

structural proteomics

protein-RNA

RNA-protein

protein-RNA complex

RNA-protein complex

CLIR-MS

Alexander Leitner
contact affiliation	ETH Zurich, Institute of Molecular Systems Biology
contact email	leitner@imsb.biol.ethz.ch
lab head
Christopher Sarnowski
contact affiliation	Institute of Molecular Systems Biology, Department of Biology, ETH Zürich
contact email	sarnowski@imsb.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD031381

PRIDE project URI

• PRIDE
  1. PXD031381
     1. Label: PRIDE project
     2. Name: Structural requirements for photo-induced RNA-protein cross-linking