PXD031372-1

PXD031372 is an original dataset announced via ProteomeXchange.

Title	Hyperammonemia-dependent and independent skeletal muscle phosphoproteomic responses during exercise
Description	Phosphorylation of skeletal muscle proteins mediates cellular signaling and adaptive responses to exercise. Bioinformatic and machine learning approaches identified preclinical models that recapitulate human exercise responses. Feature selection showed that muscles from treadmill running mice and maximum intensity contractions shared the most differentially phosphorylated phosphosites (DPPS) with human exercise. Benefits of exercise in chronic diseases may be reduced by hyperammonemia, a consistent perturbation in chronic diseases and a muscle cytotoxin generated during contractile activity. Comparative analysis of experimentally validated molecules identified 63 DPPS on 265 differentially expressed phosphoproteins (DEpP) shared between hyperammonemia in myotubes and skeletal muscle from exercise models. Functional enrichment analyses revealed distinct temporal patterns of enrichment shared between hyperammonemia and exercise models including protein kinase A(PKA), calcium signaling, mitogen activated protein kinase(MAPK) signaling, and protein homeostasis. Our approach of feature extraction of comparative unbiased data allows for model selection and target identification to optimize responses to interventions.
HostingRepository	PRIDE
AnnounceDate	2022-10-25
AnnouncementXML	Submission_2022-10-25_13:51:55.239.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031372
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	LingLi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-01 01:24:59	ID requested
⏵ 1	2022-10-25 13:51:55	announced

10.6019/PXD031372;

submitter keyword: mass spectrometry,Hyperammonemia, skeletal muscle, phosphoproteomics

SrinivasanDasarathy
contact affiliation	Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA
contact email	dasaras@ccf.org
lab head
LingLi
contact affiliation	Cleveland Clinic
contact email	lil5@ccf.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031372
     1. Label: PRIDE project
     2. Name: Hyperammonemia-dependent and independent skeletal muscle phosphoproteomic responses during exercise