PXD031362 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | eIF4A3 interactome by IP-MS in mouse ESCs |
Description | RNA helicases are involved in multiple steps of RNA metabolism to direct their roles in gene expression, yet their functions in pluripotency control remain largely unexplored. Starting from an RNAi screen of RNA helicases, we identified that eIF4A3, a DEAD-box (Ddx) helicase component of the exon junction complex (EJC), is essential for the maintenance of embryonic stem cells (ESCs). We mapped the eIF4A3 interactomes in mouse ESCs, revealing that eIF4A3 is widely involved in the post-transcriptional regulation of gene expression. Mechanistically, we show that eIF4A3 post-transcriptionally controls the pluripotency-related cell cycle regulators and that its depletion causes cellular differentiation via cell cycle dysregulation. Specifically, eIF4A3 is required for the efficient nuclear export of Ccnb1 mRNA, which encodes Cyclin B1, a key component of the pluripotency-promoting pathway during cell cycle progression of ESCs. Our results reveal a previously unappreciated role of eIF4A3 and its associated EJC in the post-transcriptional cell cycle control in maintaining stem cell pluripotency. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:42:02.081.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xin Huang |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-01-31 02:05:24 | ID requested | |
1 | 2023-03-11 03:36:48 | announced | |
⏵ 2 | 2023-11-14 08:42:04 | announced | 2023-11-14: Updated project metadata. |
Publication List
Li D, Yang J, Malik V, Huang Y, Huang X, Zhou H, Wang J, An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity. Nucleic Acids Res, 50(21):12462-12479(2022) [pubmed] |
Keyword List
submitter keyword: IP-MS,eIF4A3, ESCs |
Contact List
Jianlong Wang |
contact affiliation | Department of Medicine, Columbia University Irving Medical Center |
contact email | jw3925@cumc.columbia.edu |
lab head | |
Xin Huang |
contact affiliation | Department of Medicine, Columbia University Irving Medical Center |
contact email | xh2200@cumc.columbia.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD031362 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031362
- Label: PRIDE project
- Name: eIF4A3 interactome by IP-MS in mouse ESCs