PXD031362

PXD031362 is an original dataset announced via ProteomeXchange.

Title	eIF4A3 interactome by IP-MS in mouse ESCs
Description	RNA helicases are involved in multiple steps of RNA metabolism to direct their roles in gene expression, yet their functions in pluripotency control remain largely unexplored. Starting from an RNAi screen of RNA helicases, we identified that eIF4A3, a DEAD-box (Ddx) helicase component of the exon junction complex (EJC), is essential for the maintenance of embryonic stem cells (ESCs). We mapped the eIF4A3 interactomes in mouse ESCs, revealing that eIF4A3 is widely involved in the post-transcriptional regulation of gene expression. Mechanistically, we show that eIF4A3 post-transcriptionally controls the pluripotency-related cell cycle regulators and that its depletion causes cellular differentiation via cell cycle dysregulation. Specifically, eIF4A3 is required for the efficient nuclear export of Ccnb1 mRNA, which encodes Cyclin B1, a key component of the pluripotency-promoting pathway during cell cycle progression of ESCs. Our results reveal a previously unappreciated role of eIF4A3 and its associated EJC in the post-transcriptional cell cycle control in maintaining stem cell pluripotency.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:42:02.081.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xin Huang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-31 02:05:24	ID requested
1	2023-03-11 03:36:48	announced
⏵ 2	2023-11-14 08:42:04	announced	2023-11-14: Updated project metadata.

Li D, Yang J, Malik V, Huang Y, Huang X, Zhou H, Wang J, An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity. Nucleic Acids Res, 50(21):12462-12479(2022) [pubmed]

submitter keyword: IP-MS,eIF4A3, ESCs

Jianlong Wang
contact affiliation	Department of Medicine, Columbia University Irving Medical Center
contact email	jw3925@cumc.columbia.edu
lab head
Xin Huang
contact affiliation	Department of Medicine, Columbia University Irving Medical Center
contact email	xh2200@cumc.columbia.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD031362

PRIDE project URI

• PRIDE
  1. PXD031362
     1. Label: PRIDE project
     2. Name: eIF4A3 interactome by IP-MS in mouse ESCs