<<< Full experiment listing


PXD031355 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA Bacterial Mannose Binding Lectin as a Tool for the Enrichment of C-and O- Mannosylated Peptides
DescriptionC-mannosylated peptides are easily detected by mass spectrometry (MS) from purified proteins but not from complex biological samples. Enrichment of specific glycopeptides by lectin affinity prior to MS analysis has been widely applied to support glycopeptide identification, but was up till now not available for C-mannosylated peptides. Here we used the α-mannose-specific Burkholderia cenocepacia lectin A (BC2L-A) and show that, in addition to its previously demonstrated high-mannose N-glycan binding capability, this lectin is able to retain C- and O-mannosylated peptides. Next to testing binding abilities to standard peptides, we applied BC2L-A affinity to enrich C-mannosylated peptides from complex samples of tryptic digests of HEK293 and MCF10A whole cell extracts, which led to the identification of novel C-mannosylation sites. In conclusion, BC2L-A enabled specific enrichment of C- and O-mannosylated peptides and might have superior properties over other lectins for binding to α-mannose-terminating glycans.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAdnan Halim
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListO-mannosyl-L-threonine; 1'-mannosyl-L-tryptophan; monohydroxylated residue; O-mannosyl-L-serine
InstrumentOrbitrap Fusion Lumos; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-31 01:11:47ID requested
12022-05-13 08:13:42announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: BC2L-A lectin, C-Mannosylation, O-Mannosylation, ETciD, Glycoproteomics
Contact List
Adnan Halim
contact affiliationUniversity of Copenhagen Faculty of Health Sciences Centre for Glycomics Department of Cellular and Molecular Medicine Copenhagen Denmark
contact emailhalim@sund.ku.dk
lab head
Adnan Halim
contact affiliationUniversity of Copenhagen
contact emailhalim@sund.ku.dk
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD031355
PRIDE project URI
Repository Record List
[ + ]