PXD031355

PXD031355 is an original dataset announced via ProteomeXchange.

Title	A Bacterial Mannose Binding Lectin as a Tool for the Enrichment of C-and O- Mannosylated Peptides
Description	C-mannosylated peptides are easily detected by mass spectrometry (MS) from purified proteins but not from complex biological samples. Enrichment of specific glycopeptides by lectin affinity prior to MS analysis has been widely applied to support glycopeptide identification, but was up till now not available for C-mannosylated peptides. Here we used the α-mannose-specific Burkholderia cenocepacia lectin A (BC2L-A) and show that, in addition to its previously demonstrated high-mannose N-glycan binding capability, this lectin is able to retain C- and O-mannosylated peptides. Next to testing binding abilities to standard peptides, we applied BC2L-A affinity to enrich C-mannosylated peptides from complex samples of tryptic digests of HEK293 and MCF10A whole cell extracts, which led to the identification of novel C-mannosylation sites. In conclusion, BC2L-A enabled specific enrichment of C- and O-mannosylated peptides and might have superior properties over other lectins for binding to α-mannose-terminating glycans.
HostingRepository	PRIDE
AnnounceDate	2022-05-13
AnnouncementXML	Submission_2022-05-13_08:13:41.737.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adnan Halim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	O-mannosyl-L-threonine; 1'-mannosyl-L-tryptophan; monohydroxylated residue; O-mannosyl-L-serine
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-31 01:11:47	ID requested
⏵ 1	2022-05-13 08:13:42	announced

Dataset with its publication pending

submitter keyword: BC2L-A lectin, C-Mannosylation, O-Mannosylation, ETciD, Glycoproteomics

Adnan Halim
contact affiliation	University of Copenhagen Faculty of Health Sciences Centre for Glycomics Department of Cellular and Molecular Medicine Copenhagen Denmark
contact email	halim@sund.ku.dk
lab head
Adnan Halim
contact affiliation	University of Copenhagen
contact email	halim@sund.ku.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031355
     1. Label: PRIDE project
     2. Name: A Bacterial Mannose Binding Lectin as a Tool for the Enrichment of C-and O- Mannosylated Peptides