PXD031249

PXD031249 is an original dataset announced via ProteomeXchange.

Title	Spatial-region-resolved proteomic map reveals mechanism of COVID-19-associated heart injury
Description	Coronavirus disease 2019 (COVID-19) induces diverse clinical manifestations accompanied by multi-organ dysfunction. Of these, heart complications are the most life threatening. Direct myocardial and vascular injuries due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) drive systemic inflammation, which is the leading cause of acute cardiac injury associated with COVID-19. However, in-depth knowledge of the injury characteristics and quantity of its constituent proteins, as well as specific functions of four heart regions, including two ventricles and atria, is lacking. Here, through a strategy for spatial quantitative proteomics by combining comparative anatomy, laser-capture microdissection, and mass spectrometry, we successfully established a complete region-resolved heart proteome map by myocardia and microvessels from four regions of the hearts. We analyzed the dysfunction of different regions of COVID-19 patients’ native heart tissues in situ with histological examinaton. Focusing on the molecular features of myocardial and microvessel tissues with obvious inflammatory cells, a series of specific molecular dysfunctions of myocardium and microvessel were located in different cardiac regions caused by inflammation. These results could provide guidance for future discovery of improved clinical treatments for heart diseases.
HostingRepository	iProX
AnnounceDate	2022-01-26
AnnouncementXML	Submission_2022-10-09_00:09:04.414.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ling Leng
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-25 23:31:57	ID requested
1	2022-05-15 05:57:04	announced
⏵ 2	2022-10-09 00:09:05	announced	2022-10-09: Update publication information.

Leng L, Ma J, Zhang PP, Xu SC, Li X, Jin Y, Cai J, Tang R, Zhao L, He ZC, Li MS, Zhang H, Zhou LR, Wu ZH, Li TR, Zhu YP, Wang YJ, Wu HB, Ping YF, Yao XH, Zhu CH, Guo HT, Tan LY, Liang ZY, Bian XW, Zhang SY, Spatial region-resolved proteome map reveals mechanism of COVID-19-associated heart injury. Cell Rep, 39(11):110955(2022) [pubmed]

submitter keyword: COVID-19, SARS-CoV-2, myocardia, microvessels, heart, proteomics

Ling Leng
contact affiliation	Peking Union Medical College and Chinese Academy of Medical Sciences
contact email	zhenlinger@126.com
lab head
Ling Leng
contact affiliation	Peking Union Medical College and Chinese Academy of Medical Sciences
contact email	zhenlinger@126.com
dataset submitter

iProX dataset URI